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Aesculin Blood Agar
Aesculin Blood Agar Aesculin Blood Agar
Enterococcus faecalis on Aesculin Blood Agar
The same agar examined with ultra violet light. Fluorescence is absent in the area immediately around the streak due to aesculin hydrolysis.
The purpose of this test is to determine whether an organism can hydrolyse the glycoside aesculin to aesculetin and glucose. Aesculin fluoresces under ultra violet light, but fluorescence is lost when the glycoside is hydrolysed.

The pictures below show Streptococcus agalactiae on Aesculin Blood Agar. This bacterium does not hydrolyse aesculin.

Click on the pictures to see enlarged versions.
© Department of Veterinary Disease Biology 2011
Faculty of Health and Medical Sciences - University of Copenhagen